Protein-coding gene in the species Homo sapiens
| NDC80 |
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| Available structures |
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| PDB | Ortholog search: PDBe RCSB |
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| List of PDB id codes |
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2IGP, 2VE7, 3IZ0 |
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| Identifiers |
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| Aliases | NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hskinetochore complex component, NDC80 kinetochore complex component |
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| External IDs | OMIM: 607272; MGI: 1914302; HomoloGene: 38141; GeneCards: NDC80; OMA:NDC80 - orthologs |
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| Gene location (Human) |
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 | | Chr. | Chromosome 18 (human)[1] |
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| | Band | 18p11.32 | Start | 2,571,557 bp[1] |
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| End | 2,616,635 bp[1] |
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| Gene location (Mouse) |
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 | | Chr. | Chromosome 17 (mouse)[2] |
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| | Band | 17|17 E1.3 | Start | 71,803,095 bp[2] |
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| End | 71,833,852 bp[2] |
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| RNA expression pattern |
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| Bgee | | Human | Mouse (ortholog) |
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| Top expressed in | - ventricular zone
- ganglionic eminence
- gonad
- testicle
- trabecular bone
- amniotic fluid
- bone marrow
- secondary oocyte
- sperm
- seminal vesicula
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| | Top expressed in | - zygote
- secondary oocyte
- tail of embryo
- genital tubercle
- primary oocyte
- spermatocyte
- ventricular zone
- epiblast
- maxillary prominence
- mandibular prominence
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| | More reference expression data |
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| BioGPS |  | | More reference expression data |
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| Gene ontology |
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| Molecular function | - structural constituent of cytoskeleton
- protein binding
- identical protein binding
| | Cellular component | - cytosol
- membrane
- chromosome
- Ndc80 complex
- chromosome, centromeric region
- nucleus
- kinetochore
- nucleoplasm
- centrosome
| | Biological process | - chromosome segregation
- mitotic spindle organization
- cell division
- establishment of mitotic spindle orientation
- mitotic sister chromatid segregation
- cell cycle
- attachment of spindle microtubules to kinetochore
- sister chromatid cohesion
- metaphase plate congression
- attachment of mitotic spindle microtubules to kinetochore
- positive regulation of mitotic cell cycle spindle assembly checkpoint
- mitotic cell cycle
- kinetochore organization
- positive regulation of protein localization to kinetochore
| | Sources:Amigo / QuickGO |
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| Wikidata |
| View/Edit Human | View/Edit Mouse |
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Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene.[5][6][7]
Function
Ndc80 is one of the proteins of outer kinetochore. It forms a heterotetramer with proteins NUF2, SPC25,[8] and SPC24. This protein complex has microtubule-binding domains.[9]
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[7]
Interactions
NDC80 has been shown to interact with MIS12,[10][11] NEK2[12][13] and PSMC2.[13]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000080986 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024056 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Chen Y, Riley DJ, Chen PL, Lee WH (October 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Molecular and Cellular Biology. 17 (10): 6049–56. doi:10.1128/mcb.17.10.6049. PMC 232454. PMID 9315664.
- ^ Martin-Lluesma S, Stucke VM, Nigg EA (September 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267–70. Bibcode:2002Sci...297.2267M. doi:10.1126/science.1075596. PMID 12351790. S2CID 7879023.
- ^ a b "Entrez Gene: NDC80 NDC80 homolog, kinetochore complex component (S. cerevisiae)".
- ^ Human kinetochore protein Spc25 Q9HBM1
- ^ D'Archivio S, Wickstead B (February 2017). "Trypanosome outer kinetochore proteins suggest conservation of chromosome segregation machinery across eukaryotes". The Journal of Cell Biology. 216 (2): 379–391. doi:10.1083/jcb.201608043. PMC 5294786. PMID 28034897.
- ^ Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (September 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
- ^ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (November 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821. S2CID 39408000.
- ^ Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (December 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
- ^ a b Chen Y, Sharp ZD, Lee WH (September 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
Further reading
- Chen Y, Sharp ZD, Lee WH (September 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". The Journal of Biological Chemistry. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
- Zheng L, Chen Y, Lee WH (August 1999). "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins". Molecular and Cellular Biology. 19 (8): 5417–28. doi:10.1128/mcb.19.8.5417. PMC 84384. PMID 10409732.
- Zheng L, Chen Y, Riley DJ, Chen PL, Lee WH (May 2000). "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p". Molecular and Cellular Biology. 20 (10): 3529–37. doi:10.1128/MCB.20.10.3529-3537.2000. PMC 85645. PMID 10779342.
- Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (December 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". The Journal of Biological Chemistry. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
- Tien AC, Lin MH, Su LJ, Hong YR, Cheng TS, Lee YC, Lin WJ, Still IH, Huang CY (January 2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". Molecular & Cellular Proteomics. 3 (1): 93–104. doi:10.1074/mcp.M300072-MCP200. PMID 14602875.
- DeLuca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED (December 2003). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Current Biology. 13 (23): 2103–9. Bibcode:2003CBio...13.2103D. doi:10.1016/j.cub.2003.10.056. PMID 14654001. S2CID 16183757.
- Bharadwaj R, Qi W, Yu H (March 2004). "Identification of two novel components of the human NDC80 kinetochore complex". The Journal of Biological Chemistry. 279 (13): 13076–85. doi:10.1074/jbc.M310224200. PMID 14699129.
- Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (May 2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". The Journal of Biological Chemistry. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
- Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M (April 2004). "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo". Current Biology. 14 (7): 611–7. Bibcode:2004CBio...14..611J. doi:10.1016/j.cub.2004.03.031. PMID 15062103. S2CID 13757802.
- Steensgaard P, Garrè M, Muradore I, Transidico P, Nigg EA, Kitagawa K, Earnshaw WC, Faretta M, Musacchio A (June 2004). "Sgt1 is required for human kinetochore assembly". EMBO Reports. 5 (6): 626–31. doi:10.1038/sj.embor.7400154. PMC 1299074. PMID 15133482.
- Stucke VM, Baumann C, Nigg EA (August 2004). "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1". Chromosoma. 113 (1): 1–15. doi:10.1007/s00412-004-0288-2. PMID 15235793. S2CID 28231914.
- Meraldi P, Draviam VM, Sorger PK (July 2004). "Timing and checkpoints in the regulation of mitotic progression". Developmental Cell. 7 (1): 45–60. doi:10.1016/j.devcel.2004.06.006. PMID 15239953.
- Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (September 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
- Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (November 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821. S2CID 39408000.
- Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC (January 2005). "Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling". The Journal of Biological Chemistry. 280 (3): 2092–104. doi:10.1074/jbc.M403080200. PMID 15504738.
- DeLuca JG, Dong Y, Hergert P, Strauss J, Hickey JM, Salmon ED, McEwen BF (February 2005). "Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites". Molecular Biology of the Cell. 16 (2): 519–31. doi:10.1091/mbc.E04-09-0852. PMC 545888. PMID 15548592.
2igp: Crystal Structure of Hec1 CH domain
